What are the methods for determining amylose?

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We all have the common sense of life, that is, in the roots, stems or seeds of plants, starch is a widely-existing substance, but few people know what the composition of starch is. In fact, the starch composition is divided into two types of amylose and amylopectin. The ratio of amylose to amylopectin in natural starch is generally about 15-28% to 72-85%, depending on the plant species, variety, and growth period. Different, the ratio is different, and the quality characteristics are also very different. In the field of modern agriculture, the value of amylose is gradually recognized by people. Therefore, it is clear that amylose content has important practical significance in evaluating food quality and breeding and breeding. So what are the methods for measuring amylose ?

At present, the methods for determining amylose content mainly include iodine colorimetry and iodine affinity titration. In addition, there are new methods such as chromatographic analysis, near-infrared spectroscopy, automatic analysis and detection, and concanavalin method. The current effective national standards for amylose detection are: GB/T 15683-2008 Determination of amylose content in rice, NY/T 2639-2014 Determination of rice amylose by spectrophotometry, DB32/T 2265-2012 Determination of amylose and amylopectin content in corn -- Two-wavelength spectrophotometric method, DB63/T 1671-2018 Determination of amylose and amylopectin content in barley. Spectrophotometry, GB 7648-1987 Rice, corn, millet grain Amylose assay.

Because the traditional amylose determination method is complicated, the operation steps are cumbersome and the efficiency is low, the detection and analysis threshold of amylose such as rice is high, and the selection of agricultural agriculture and breeding is restricted, and the technology is The development and research and development of professional instruments for rapid testing and analysis of amylose content are becoming more and more demanding. Under this background, amylose analyzers have emerged as the times require, which satisfies the practical needs of modern agriculture for rapid detection and screening of rice quality.

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